How much automation are you able to incorporate into your perfusion processes for production? Can it be a fully automated or close to fully automated process?

Question

Accepted Answer

The industry is working toward solutions that enable fully automated processes that will allow better control and improve robustness of the process. Automation includes various components. Some of them are well established and routinely used in perfusion, such as capacitance probes. Other components that are part of perfusion systems require additional work to be integrated with the bioreactor systems. For example, automated cell bleeding can be achieved by integrating a continuous pump with an on-line cell density probe. For production perfusion, advanced automation tools are being developed to monitor product quality as well. A collaborative approach is needed between vendors and facility owners to achieve full automation of N-1 and perfusion processes.

Answer

For seed train expansion, do you find that you need a more complex media with perfusion? Have you found any supplements that are very useful to support high densities?

We are having difficulty in maintaining a constant working volume in our production bioreactors. We have been trying to balance the harvest and feed pumps at a consistent perfusion rate, but we keep seeing that this is not maintaining the same working volume.

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