We are having difficulty in maintaining a constant working volume in our production bioreactors. We have been trying to balance the harvest and feed pumps at a consistent perfusion rate, but we keep seeing that this is not maintaining the same working volume.
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Utilizing N-1 Perfusion to Increase Productivity and Intensify Processes
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Company: Catalent Biologics
Job Title: Director, Process Development Department
Answer
Different factors can contribute to challenges to maintain constant volume, including pump accuracy. When pumps are operating for long periods of time, calibration may be compromised and may need a flow sensor. Controlling working volume weight is a good alternative that can be achieved by having a weight cell in the bioreactor.
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