How to avoid mold contamination during hybridoma culture? Thanks in advance

Question

Accepted Answer

First, as in the first question above I would track down the source of the contamination.  Mold can come from many places, including workers and heating /cooling vents.  Look for the source.  Does your mold contamination carry through from the original isolation of cells from mice>  If you want you can use an antimycotic/antibiotic, like Gibco Anti/Anti.

Answer

I sometimes have a contamination in the culture bottles (usually fungus). However, when I examine all the used media I do not find any contamination in these media.

Is there any advantage in terms of volumetric productivity when a Mab cloning design includes light and heavy chain sequences in a bicistronic vector instead of two different ones?

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