Is there any advantage in terms of volumetric productivity when a Mab cloning design includes light and heavy chain sequences in a bicistronic vector instead of two different ones?
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You know I don’t really know and that answer would be based on so many things including vector, promotors, cell type, media. culture conditions to name a few things, a simple answer is not possible. I suspect there may not be much difference though. In looking around to help me answer this question I did find one patent for dual expression in e. coli.
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