How to avoid mold contamination during hybridoma culture? Thanks in advance

Answer

First, as in the first question above I would track down the source of the contamination. Mold can come from many places, including workers and heating /cooling vents. Look for the source. Does your mold contamination carry through from the original isolation of cells from mice> If you want you can use an antimycotic/antibiotic, like Gibco Anti/Anti.