I am getting ready to move my ES cells from MEF cells to Matrigel matrix. Do you have any recommendations for the best method, and are there any special requirements for the media?
This question is part of the following Ask The Expert session:
Everything you ever wanted to ask about Corning® Matrigel® Matrix
Job Title: Technology Manager
Corning Matrigel hESC-qualified matrix has been used extensively as a substrate for culturing human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSCs) with numerous hPSC culture media such as mTeSR™1, TeSR™2, E8, NutriStem®, and MEF conditioned media.
Corning has published a protocol describing the use of Corning® Matrigel® hESC-qualified matrix-coated 6-well plate with mTeSR™1 media for hESC culture. This protocol may be adapted to other media as well. Results may vary depending upon the cell line used, media, and dissociation technique, etc. You should optimize conditions for your own system.
Transitioning hPSCs from mouse embryonic fibroblasts (MEFs) to Matrigel matrix does not typically require any special process steps. Cells can be plated in medium of your choice on Matrigel hESC-qualified matrix-coated vessels at the time of passage. You should optimize your passaging conditions based on the cell line used, media, and dissociation technique. You can watch a video on how to passage ES cells from MEFs to Matrigel hESC-qualified matrix on the Journal of Visual Experiments (JoVE) web site.
For further support or troubleshooting advice, feel free to reach to the Corning Scientific Support team.