My reprogramming experiment is at Day 21 post induction and I am ready to select colonies for clonal expansion, how do I know which to pick?

Answer

At the time of selecting colonies to expand, the use of morphology can also be aided by pluripotent markers such as Alkaline Phosphatase Live Stain, which can be used to select robustly, uniformly expressing clones. The dye can be used to transiently identify fully reprogrammed colonies without affecting survival or cell growth once transferred to new culture dishes.