If you want to include a low level of antibiotic in your hybridoma cell culture to reduce the risk of contamination, which type would you recommend and how much?
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Antibiotics in Cell Culture – What types are available and when and how to utilize them
Company: BioTechnical Institute of Maryland
Job Title: Director
My first thought would be penicillin and streptomycin at 50 ug each/ml. The two can be purchased supplied together and is called pen-strep. Penicillin kills gram positive bacteria by inhibiting cell wall synthesis and streptomycin kills by inhibiting protein synthesis in gram positive and gram negative bacteria. If you are using a serum-free medium use 25 ug/ml of each.