If you want to include a low level of antibiotic in your hybridoma cell culture to reduce the risk of contamination, which type would you recommend and how much?


My first thought would be penicillin and streptomycin at 50 ug each/ml. The two can be purchased supplied together and is called pen-strep. Penicillin kills gram positive bacteria by inhibiting cell wall synthesis and streptomycin kills by inhibiting protein synthesis in gram positive and gram negative bacteria. If you are using a serum-free medium use 25 ug/ml of each.

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