Which are all the antibiotics can be used in combination in large scale Vero cell culture and at what concentration ?
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Antibiotics in Cell Culture – What types are available and when and how to utilize them
Company: BioTechnical Institute of Maryland
Job Title: Director
This question can be answered many ways depending on the goals. Maybe you would like to be proactive and you want to use antibiotics to prevent an infection or maybe you want to cure a current infection. Of course my recommendation is not to use antibiotics unless absolutely necessary. I prefer having good frozen stocks of cells that I can thaw if needed. Having said that though, I suspect the best general antimycotic is Fungizone (Amphptericin B). At normal working concentrations (0.5-2.5 ug/ml) it works to prevent fungal contaminations. Interestingly in Vero cells, at low concentrations (250 ng/ml) amphotericin B has been show to increase viral uptake (http:/www.ncbi.nih.gov/pmc/articles/PMC3194987/). I have provided a listing below of other antibiotics and antimycotics and their working concentrations. I will mention that the range of antibiotics and antimycotics used does vary depending of the media type used. Media containing serum can contain more antibiotic/antimycotic since the high protein, mostly albumin binds much of the reagent. If you are using a low protein medium or a serum-free medium you will need to use much less for equivalent results, perhaps 10% to 50% depending on specifics of your culture method.
NAME , CONCENTRATION, SPECTRUM, STABILITY AT 37C IN MEDIA
Anti-PPLO Agent , 10-100 ug/ul, Mycoplasma and gram positive bacteria, 3 days
Fungizone, 0.25-2.5 ug/ul, Fungi and yeast, 3 days
Gentamicin Sulfate, 5-50 ug/ml, Gram positive and gram negative bacteria and mycoplasma, 3 days
Kanamcin Sulfate, 100 ug/ml, Gram positive and gram negative bacteria and mycoplasma, 5 days
Neomycin Sulfate, 50 ug/ml, Gram positive and gram negative bacteria, 5 days
Nystatin, 100 U/ml, Fungi and yeast, 3 days
Penicillin G, 50-100 U/ml, Gram positive bacteria, 3 days
Polymixin B Sulfate, 100 U/ml, Gram negative bacteria, 5 days
Streptomycin Sulfate, 50-100 ug/ml, Gram positive and gram negative bacteria, 3 days