I have selected my reprogrammed colonies and am expanding them, how can I further confirm that they are still pluripotent?

Answer

The continued proliferation of the clones with good morphology is a good indicator, but further characterization is necessary. The continued expression of self-renewal markers, such as SSEA4, Tra-1-60 and Tra-1-81, can be supplemented by looking at intracellular markers  of self-renewal like OCT4, SOX2, KLF4 and Nanog via antibody staining.

For a complete list of PSC markers, please visit lifetechnologies.com/stemcellantibodies