I am looking for an efficient and easy way to isolate B cells from whole blood without using magnetic beads. Any suggestions?
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The first step in B cell isolation is to isolate mononuclear cells from whole blood through centrifugation with a Ficoll gradient. Here is a protocol for reference that uses Corning Lymphocyte Separation Medium as part of this process. There are many different B cell isolation kits that are commercially available, however, you will find it difficult to find one that does not use magnetic beads as part of the isolation process.
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