I am looking for an efficient and easy way to isolate B cells from whole blood without using magnetic beads. Any suggestions?

Answer

The first step in B cell isolation is to isolate mononuclear cells from whole blood through centrifugation with a Ficoll gradient. Here is a protocol for reference that uses Corning Lymphocyte Separation Medium as part of this process. There are many different B cell isolation kits that are commercially available, however, you will find it difficult to find one that does not use magnetic beads as part of the isolation process.

Pin It on Pinterest