What are minimal criteria when we do NOT need to use Poloxamer (cell concentration, tip speed, bioreactor size, pore sparger size, metabolic activity, etc)
This question is part of the following Ask The Expert session:
Addressing Shear Stress in Bioreactors
Answered by:
Company: Merck
Job Title: Head of Perfusion Systems Lab, Life Science, BioProcessing R&D,
Answer
We have not investigated this in detail. Due to cell specific sensitivity differences, one cannot make general comments to this question. And due to the effectiveness of Poloxamer 188 this is an unnecessary risk.
This post is sponsored by MilliporeSigma.