Do we need Poloxamer 188 in seed train?
This question is part of the following Ask The Expert session:
Addressing Shear Stress in Bioreactors
Job Title: Head of Perfusion Systems Lab, Life Science, BioProcessing R&D,
Yes, Poloxamer 188 in seed trains is needed to adapt the cells to the media composition and to protect the cells from shear. Especially after thaw, cells are very sensitive.