What are minimal criteria when we do NOT need to use Poloxamer (cell concentration, tip speed, bioreactor size, pore sparger size, metabolic activity, etc)
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Addressing Shear Stress in Bioreactors
Job Title: Head of Perfusion Systems Lab, Life Science, BioProcessing R&D,
We have not investigated this in detail. Due to cell specific sensitivity differences, one cannot make general comments to this question. And due to the effectiveness of Poloxamer 188 this is an unnecessary risk.