What are minimal and maximal concentrations of Poloxamer recommended? Any correlations with cell mass?
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Addressing Shear Stress in Bioreactors
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Company: Merck
Job Title: Head of Perfusion Systems Lab, Life Science, BioProcessing R&D,
Answer
This is mainly cell line dependent – we saw effective protection between 0,5-2 g/L in our stress test, which is of course a simplified model. According to literature, concentrations up to 5 g/l have been used for very stressful conditions (high VCD Perfusion), providing protection without any negative side effects. As a minimum we have also seen very few customers using <0,5g/L Poloxamer 188 in their processes.
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