My iPSC have demonstrated their self-renewal and differentiation potential, but how do I know they measure up to other established ESCs or iPSCs?
This question is part of the following Ask The Expert session:
Options for Identification and Characterization of Pluripotent Stem Cells
Answered by:
Answer
An alternative to looking at marker expression via antibody staining is to look at transcription of genes associated with self-renewal and differentiation potential. The Taqman® hPSC Scorecard™ Assay allows you to determine this through a panel of self-renewal and pluripotency markers. The panel enables qPCR analysis of either undifferentiated or differentiated cells with a single assay. It can tell you whether your differentiated iPSC have the hallmark self-renewal gene expression profile and whether your differentiated samples have tri-lineage potential or skew towards particular lineages. In addition, the cloud-based software compares your results against a panel of established reference cell lines.
Learn more about the hPSC Scorecard assay and software
This post is sponsored by Thermo Fisher Scientific, Inc..