We are having a hard time culturing primary multiple myeloma CD138+ cells (Isolate from patient bone marrow) and maintaining decent viability in 24-48h. We added 15% patient serum in medium and viability improved a little but some sample still do not have great in viability (30-50% dead in 24h).Would you give me some advice to improve viability?

Answer

This is a difficult question to answer without more information. The low viability may de due to the isolation process or/and the culture process. Below is a link to an article in the journal Blood that you may find helpful.
http://bloodjournal.hematologylibrary.org/content/103/8/3175.long