We are having a problem with our newly suspended cells clumping together. Any thoughts?
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Cell Culture Basics – Avoiding contamination, cell growth and passaging, and proper storage techniques
Company: BioTechnical Institute of Maryland
Job Title: Director
If your adherent cells that were adapted to grow in suspension are clumping you might increase the shaking speed a little bit. You can add media that is calcium and magnesium free. Sometimes I have had clumping and when I triturated the cells the clumps they broke up. Since I don’t know your culture conditions it is difficult to help more. One trick is to put the cells in a tall sterile tube like a sterile graduated cylinder and let the clumps settle for a few minutes then use the clump-free upper layer to passage the cells.