We are having an issue with foaming. Could you provide some suggestions? I’d like to avoid using antifoam because of clogging issues.
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Utilizing N-1 Perfusion to Increase Productivity and Intensify Processes
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Company: Catalent Biologics
Job Title: Director, Process Development Department
Answer
Bioreactor spargers and conditions may impact foaming. The amount of antifoam can be minimized by optimizing mass transfer conditions including P/V and gassing strategy and sparger type and/or size of sparger pores. Max challenge studies can be performed to understand the effect of antifoam in the culture and clogging. The filter can be regularly changed when fouling is detected through changes in permeate flow pressure.
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