What do you recommend for imaging of non-adherent cells?
This question is part of the following Ask The Expert session:
Fluorescence detection in living cells and ways to improve your image
Company: BioTechnical Institute of Maryland
Job Title: Director
You can do imaging on suspension cells. If you are using phase-contrast you won’t get much since the light reflects off of the cells in suspension, but you can get images using other types of microscopy. Confocal and fluorescent imaging is possible. Often fixations are necessary. The trick is using an ultra-thin layer of cells and short working distance. If you do a search on the internet for whatever imaging you want to do and use the word “suspension” you will find a protocol.