Options for Increasing Output and Reducing Footprint in Adherent Cell Culture

Sponsored by: Pall Life Sciences
Session ends: June 20th, 2014, 3:00pm MST
Answers by: Jose Castillo, Director of Cell Culture Technologies for Pall Life Sciences


Adherent cell culture is often used in the manufacture of biologic products, including vaccines. Historically, the surface for cells to adhere to has been provided either by a two-dimensional (2D) system such as roller bottles, T-flasks, cell factories or cell stacks; or microcarrier beads within a traditional stirred tank bioreactor. However the need to increase product production and reduce manufacturing footprints have led to the creation of novel solutions.

One alternative solution to traditional 2D systems and to a microcarrier-stirred tank option is a fixed-bed disposable bioreactor, which can provide efficient scale-up and manufacturing with a small footprint.

Don’t miss this chance to have your adherent cell culture questions answered!

Questions & Answers

We are interested in knowing a better method for increasing output in adherent cell culture. We have two conditions to comply: 1) We are interested in cells and not secreted products from cells 2) We need intact cells after harvest

Our Xpansion™ bioreactor would be your best option in this case, because it was specifically designed to grow and recover the cells. It was developed using advanced cell cube technology and has been built with sensors, mixers and integrated process controls to grow cells. This is the technology many of our customers are using for […]» Read More

Is there an upper limit to the volume of production for a fixed-bed system? Could this be used for large-scale production; if so, do you lose some of the footprint/cost benefits?

We currently work with an upper limit of 25L (replacing up to 6,000 roller bottles), and have found that this more than accommodates most customers’ applications in the human and animal vaccine business. In other words, it is not a limitation. In reality, there is a geometric limitation on how large it could be. While […]» Read More