Fluorescence detection in living cells and ways to improve your image

By on December 9, 2013
Ask the Expert

Fluorescence imaging of living cells can provide important data regarding the function and localization of proteins and other bio-molecules within a cell or tissue.

These images give insight into fundamentally important biological processes and improve our knowledge about transient interactions we might not be able to detect otherwise.  An added benefit is some simply remarkable pictures of colorized cells which are just fun to look at.  Although in theory, fluorescence microscopy is simple, obtaining suitable images is difficult.  Problems with cell health can occur due to long incubations in D-PBS in an attempt to reduce auto-fluorescence.  Cell death due to light intensity or photo-bleaching can be problematic and need to be overcome.  If you are having problems with signal:noise  or cell health or obtaining the best image possible now is your chance to ask the expert.

Ask a Question or View the Answers

Don’t miss this chance to have your cell imaging questions answered and get the chance to win a free set of six spray bottles for your lab, courtesy of Life Technologies!


This Ask the Expert Session is Sponsored by Life Technologies and hosted by Timothy Fawcett, Ph.D.  Dr. Fawcett has been in the biotechnology business for over 30 years.  Trained as a biochemist he has held senior positions in both academics and industry and has been a mentor to many young scientists throughout his career.  For the last 12 years Dr. Fawcett has been the Director of the BioTechnical Institute of Maryland (BTI) a non-profit institute located in Baltimore, Maryland.  He is also the Founder and Director of BioSciConcepts, a social venture of BTI that provides hands-on training for professional scientists in cell culture, baculovirus based expression, as well as topics such as molecular biology, PCR and real-time PCR. BioSciConcepts is an internationally recognized provider of expertise in the biological sciences and has provided consultation services to several small and large biotechnology companies.