Ask the Expert – Going tiny is the next BIG thing: Tools and Techniques for Organoid Cultures

By on October 30, 2017
Ask the Expert

In the last decade, organoid cultures have quickly become a popular way to create “mini-organs” to support the advancements in the study of organogenesis, disease modeling and subsequently the development of new therapies. Scientists are creating lab-grown miniature versions of organs that so far include kidney, liver, brain, prostate and pancreas, that more closely resemble the composition and functionality of organs.

There are many protocols, tools and techniques that can be used for organoid cultures – ranging from microplates, extracellular matrices, hydrogels, and bioprinting to microfluidics. Depending on your cell type, research area and ultimate goals, the options can seem overwhelming.

So how does one know where to start? Learning from the work that has already been done is a great place to start. We’ve assembled a team of Corning Life Science experts, who are ready to answer your organoid-related questions. Corning has over 30 years of experience in 3D cell culture and offers some of the original and most widely used 3D tools, such as Corning® Matrigel® matrix, Transwell® permeable supports and the Corning spheroid microplate.

Corning experts include Feng Li, Senior Scientist Development, Hilary Sherman, Applications Scientist, Himabindu Nandivada, Senior Development Scientist and Nitin Kulkarni, Sr. Scientific Support Specialist.  Dr. Feng Li has been working on 3D hepatic model systems for liver toxicity and disease modeling. His recent work includes establishment hepatic 3D spheroid culture procedures, testing primary human hepatocytes (PHHs) for 3D spheroid culture, and assay development for chronic liver toxicity testing and repeated-dosing with PHH spheroids in Corning ultra-low attachement spheroid microplates. Hilary Sherman is an Applications Scientist with Corning Life Sciences. She has worked with a wide variety of cell types including mammalian, insect, primary and stem cells in a vast array of applications, including 3D cultures. Dr. Nandivada has more than 10 years of experience in human pluripotent stem cell culture and material science. Dr. Kulkarni has worked in Scientific Support group for several years supporting 3D cell culture and recently presented talks and a webinar on surfaces used for organoid culturing.

Please take advantage of the opportunity to ask our expert a question and participate in a lively discussion on Tools and Techniques for Organoid Cultures.

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