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Using Intestinal Organoids for Intestinal and Stem Cell Research
Intestinal organoids provide a major advancement in the way we study intestinal and adult stem cell biology. When intestinal crypts containing LGR5+ intestinal stem cells (ISCs) are placed in a specialized medium that simulates the intestinal stem cell niche, the ISCs rapidly proliferate and differentiate into all of the major cell types found in the intestinal epithelium: Paneth cells, goblet cells, enteroendocrine cells, and enterocytes. Remarkably, these cells arrange themselves into a crypt-villus structure surrounding a functional central lumen, thus maintaining the stem cell pool while mimicking the physiology of the adult intestinal epithelium. The resulting organoids (or “mini-guts”) can be used for a variety of research applications including:
- intestinal stem cell biology
- intestinal disease
- adult stem cell biology
- gene therapy
- preclinical drug screening
- personalized medicine
As an in vitro system that is capable of providing in vivo insight, intestinal organoids signify an exciting new chapter for research in these fields. More details about this culture system and its research applications can be found in our previous post, Intestinal Organoid Culture: A Convenient and Physiologically Relevant Model for Intestinal and Stem Cell Research.
Have any questions about incorporating intestinal organoids into your research?
During this Ask the Expert session, Ryan Conder, Senior Scientist at STEMCELL Technologies, will be answering your questions about intestinal organoid culture, its research applications, and IntestiCult™ Organoid Growth Medium (Mouse), the first-ever complete, defined and serum-free medium for growing intestinal organoids from mouse intestinal crypts.
In this video Ryan provides a brief overview of the topic: